Hi Linda,
I took your signature and saved it on my computer, then made it transparent and saved it as a gif. I'm going to start a new thread with it too to see if I got rid of the white around the flowers but I wanted to see if you could see any difference in the colors between the one I saved and the one you saved.
When you save an image as a gif, how many colors do you use? Do you use customized settings or do you go with the default? Someone from the Creative Nook gave me instructions on how to save a gif and ever since I started using her settings, my gifs have looked much nicer as far as color goes. I think you're finished results have a lot to do with the settings you use.
Here's what I came up with.