Hi Melissa, I was just checking the messages and saw yours. Let's see, μM means micromoles per liter, μmol/L, and 1 L is 106 μL. If we use the dilution formula to calculate the micromolarities of A and B after dilution, we have,
A: (10 μL)(100 μM) = (500 μL)(X), X = 2 μM in A
B: Same thing; after dilution the solution will be 2 μM in B also.
So yes, if we take 1 μL of this solution, you would have the same amounts of A and B as if you took 1 μL each of separate solutions of 2 μM A and 2 μM B.
(1 μL)(2 μmol A/106 μL) = 2 X 10�? μmol of A
(1 μL)(2 μmol B/106 μL) = 2 X 10�? μmol of B
The only difference would be the volumes. From the mixture, you would have 2 X 10�? μmol of A and 2 X 10�? μmol of B in a total volume of 1 μL. If you take 1 μL from separate solutions of A and B, you would have 2 X 10�? μmol of A and 2 X 10�? μmol of B in a total volume of 1 μL + 1 μL = 2 μL.
Steve
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