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Organic : Restriction Enzyme Problem
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From: MSN Nickname·Steve·  in response to Message 4Sent: 11/18/2005 3:25 PM
At pHs in the 7-8 range using phosphate or TAE buffers, DNA has a negative charge due to the phosphate groups.  Since anions (-) will migrate toward the anode (+), DNA will migrate toward the anode at pHs normally used in the electrophoresis procedure.
 
 
Steve


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     re: Restriction Enzyme Problem   MSN Nickname·Steve·  11/18/2005 3:47 PM