|
|
 |
Reply
| |
Hi Steve, How does this look? Is there any thing else I can say to answer the question further (attached Word doc)? Thanks |
| |
 First
Previous
2-7 of 7
Next
Last
|
Reply
| |
oops! I just noticed I had the EcoRI splitting in the wrong spot on the bottom gene - should be between G and A. |
|
Reply
| |
Thanks Steve. How do we know the DNA fragments will always run toward the anode, or do we? |
|
Reply
| | From:  ·Steve· | Sent: 11/18/2005 3:25 PM |
At pHs in the 7-8 range using phosphate or TAE buffers, DNA has a negative charge due to the phosphate groups. Since anions (-) will migrate toward the anode (+), DNA will migrate toward the anode at pHs normally used in the electrophoresis procedure. Steve |
|
Reply
| | | From: ·Steve· | Sent: 11/18/2005 3:47 PM |
>> DNA has a negative charge due to the phosphate groups << On second look that was not stated very clearly... I mean the phosphate groups in the DNA of course, not because of a phosphate buffer particularly.  (Same idea as in your post about the charge of a phosphopeptide here.) |
|
Reply
| |
Thanks again Steve, that is mostly what I thought. |
|
|
First
Previous
2-7 of 7
Next
Last
|
|
|
Free Forum Hosting
Important Announcement
Return to Organic